Please check back for edits/updates to this program
March 29-31, 2023
NIH Campus, Bethesda, MD
Natcher Conference Center (Building 45 Conference Rooms E1 / E2 (next to main auditorium)
Wednesday, March 29th
4 – 6 PM Registration (Bethesda Hyatt Rooftop Lounge)
4 – 6 PM Opening Reception (Bethesda Hyatt Rooftop Lounge)
Thursday, March 30th
7 – 8 AM Coffee, Registration, and Poster Setup (room C1 / C2)
8:00 AM Welcome: Charles Chavkin & Hugo Tejeda
8:15 – 9:55
Oral Session 1: (Monitoring Dyn / KOR signaling; Chair: Niko Massaly)
8:15 AM
Chunyang Dong, Raajaram Gowrishankar, Yihan Jin, Jenny He, Huikun Wang, Nilüfer Sayar Atasoy, Rodolfo Floresgarcia, Karan Mahe, Achla Gupta, Jennifer Whistler, Ivone Gomes, Hugo Tejeda, Atasoy Deniz, Lakshmi Devi, Michael Bruchas, Matthew Banghart, & Lin Tian. Shedding Light on Neuropeptide Dynamics: Genetically Encoded Biosensors to Illuminate Cellular and Systemic Actions of Opioids. University of California, Davis, CA, USA.
8:35 AM
Michael Rappleye, Carlie Neiswanger, Selena S. Schattauer, Kandace Kimball, Adam Gordon-Fennell, Catalina A. Zamorano, Daniel C. Castro, Avi K. Matarasso, Carrie Stine, Sarah J. Wait, Justin Daho Lee, Jamison C. Siebart, Azra Suko, Netta Smith, Jeanot Muster, Kenneth A. Matreyek, Douglas M. Fowler, Garret D. Stuber, Michael R. Bruchas, Charles Chavkin, & Andre Berndt. Enlighten the neurophysiology of opioid signaling with genetically encoded sensors. University of Washington, Seattle WA, USA.
8:55 AM
Huikun Wang, Rodolfo Flores-Garcia, Hector Yarur-Castillo, Aaron Limoges, Hector Bravo-Rivera, Sanne M. Casello, Niharika Loomba, Juan Enriquez-Traba, Miguel Arenivar, Queenie Wang, Grace Or, Chunyang Dong, Lin Tian, & Hugo A. Tejeda. Prefrontal cortical dynorphin peptidergic transmission constrains threat-driven behavioral and network states. NIMH, Bethesda, MD, USA.
9:15 AM
Antony D. Abraham, Sanne M. Casello, Selena S. Schattauer, Brenden A. Wong, Grace O. Mizuno, Karan Mahe, Lin Tian, Benjamin B. Land& Charles Chavkin. Detection of endogenous dynorphin release in vivo in the mouse prefrontal cortex with the fluorescent sensor kLight1.2a Research Triangle Institute, Research Triangle Park, NC, USA.
9:35 AM
R. Gowrishankar, A. E. Elerding, S.E. Shirley, J. Van Tilburg, K. Abrera, D.J. Marcus, K. Motovilov, S.C. Piantadosi, A.A. Gordon-Fennell, C.Z. Zhou, C. Dong, L. Tian, G.D.& M.R. Bruchas Dynorphin-Kappa Opioid receptor control of amygdalo-striatal circuits for reward-seeking University of Washington, Seattle, WA, USA.
9:55 AM
Sineadh M. Conway, Chao-Cheng Kuo, Woodrow Gardiner, Rui-Ni Wu, Loc V. Thang, Graydon B. Gereau, John R. Cirrito, Carla M. Yuede, Jordan G. McCall, Ream Al-Hasani. An electrochemical approach for rapid, sensitive, and selective detection of dynorphin. Washington University School of Medicine, St. Louis, MO, USA.
10:15 AM Discussion
10:25 AM Coffee Break (with Posters in room C1 / C2)
10:45 AM – 12:00 PM
Oral Session 2: (Novel views on the Dyn / KOR system, Chair: Irwin Lucki)
10:45 AM
Marwa Mikati, Shenjian A, Sarah Rosen, Ayah Hamdan, Justin Woods, Robyn Klein, & Ream Al-Hasani Investigating the role of the Kappa Opioid Receptor in the immunomodulatory effects of fentanyl exposure and withdrawal Washington University School of Medicine in St. Louis, MO, USA.
11:05 AM
John Grothusen & Renyu Liu Salvinorin A, a natural Kappa opioid receptor agonist, for acute stroke treatment University of Pennsylvania, Philadelphia, PA, USA
11:25 AM
Abigail G. Schindler, Bryan Schuessler, Ari Asarch, Janet Lee, Britahny Baskin, Mayumi Yagi, & Mackenzie Patarino, Mapping time-dependent dynorphin/kappa opioid receptor activation to Polytrauma Clinical Triad-related outcomes following repetitive blast trauma University of Washington and VA Puget Sound, Seattle, WA, USA
11:45 AM
Travis D. Goode, Delara Chizari, Nina Sachdev, Antoine Besnard, Michael D. Kritzer, Devesh Pathak, Evan Z. Macosko, & Amar Sahay Hippocampus-Dependent Calibration of Context-Evoked Feeding by a Prodynorphin-Expressing Lateral Septum to Lateral Hypothalamus Circuit Mass. Gen. Hosp., Boston, MA, USA
12:05 AM Discussion
12:15 PM Nora Volkow, NIDA Director
12:30 PM -1:30 PM Buffet Lunch (rooms F1 / F2, G1 / G2)
1:30 PM – 2:40 PM
Oral Session 3: (Dyn / KOR and addiction, Chair: Bill Carlezon)
1:30 PM
Matthew B. Pomrenze, Daniel F. Cardozo Pinto, Peter A. Neumann, Pierre Llorach, Jason M. Tucciarone, Neir Eshel, Boris D. Heifets, Robert C. Malenka Modulation of 5-HT release by dynorphin mediates social deficits during opioid withdrawal Stanford University School of Medicine, Stanford, CA, USA
1:50 PM
Ruby A. Holland, Kelly M. Smith, Michael C. Chiang, Jeffrey Okoro, Eileen K. Nguyen, Isabel H. Bleimeister, Samantha A. Sherman, Ava V. Zoltanski, Sarah E. Ross, Kappa-opioid receptor-expressing neurons in the ventral tegmental area reverse opioid withdrawal symptoms University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
2:10 PM
Brendan M. Walker, and Gengze Wei Inducible Oprk1 knockdown in the basolateral amygdala rescues dysphoria cue-induced escalation of alcohol self-administration. University of South Florida, Tampa, FL, USA
2:30 PM Discussion
2:40 PM Coffee Break (with Posters in C1 / C2)
3:00 PM
Oral Session 4: (Molecular mechanisms, Chair: Lee Yuan Liu-Chen)
3:00 PM
Jianming Han, Jingying Zhang, Antonina Nazarova, Sarah M. Bernhard, Brian E. Krumm, Lei Zhao, Jordy Homing Lam, Vipin Rangari, Susruta Majumdar, David E. Nichols, Vsevolod Katritch, Peng Yuan, Jonathan F. Fay, Tao Che, Ligand and G Protein Selectivity in Kappa Opioid Receptor Revealed by Structural Pharmacology Washington University in St. Louis, St. Louis, MO, USA
3:20 PM
Carlie Neiswanger, Selena S. Schattauer, Kandace Kimball, Micaela V. Ruiz, Justin Lee, Andre Berndt, Charles Chavkin Kappa Receptor Partial Agonists Inactivate KOR through a JNK/ROS Mechanism University of Washington, Seattle, WA, USA
3:40 PM
Anushka Dikshit, Sayantani Basak, Laetitia Chatelain, Steve Zhou, Ching-Wei Chang, Connie Zhang, John Pulliam. Spatial mapping of pain-associated G-protein coupled receptors and biomarker localization in mouse brain using RNAscopeTM HiPlex v2 and RNA-protein Co-detection assay. Advanced Cell Diagnostics, a Bio-Techne brand, Newark, CA, USA
4:00 PM
Vladana Vukojević, Sho Oasa, Aleksandar J. Krmpot, Stanko N. Nikolić, Lars Terenius Ethanol effect on nalfurafine (NFF) binding to kappa-opioid receptor (KOP). Live cell study using Fluorescence Lifetime Imaging Microscopy (FLIM) Karolinska Institutet, Stockholm, Sweden
4:20 PM Discussion
4:30 PM – 5:30 PM Poster session (room C1 / C2)
6:00 PM – 9:00 PM Student / Postdoc Mixer
(Rock Bottom Brewery, 7900 Norfolk Ave, Bethesda, MD)
Dinner (no host, maps to local restaurants provided)
Friday, March 31st
7 – 8 AM Coffee & Registration
8:00 – 9:55 AM
Oral Session 5: (Pain, Chair: Catherine Cahill)
8:00 AM
Olayinka Idowu, Hye Jean Yoon, Rossana Sandoval, Catherine Cahill, Marco Pignatelli, Jose Moron-Concepcion, & Nicolas Massaly Dissecting the accumbal dynorphinergic outputs underlying affective pain Washington University in St. Louis and University of California, Los Angeles, CA, USA
8:20 AM
Morón-Concepcion J.A, Lorente, J.D., Campos-Jurado, Y., Ibrahim, K.M., Massaly, N., & Hipólito, L. Dissecting the role of a central amygdala to nucleus accumbens dynophinergic projection in pain-induced negative affect Washington University in St. Louis, St. Louis, MO, USA
8:40 AM
Manish K. Madasu, Loc V. Thang, Sriya Chebrolu, Priyanka Chilukuri, Fiona Bree, Tayler D. Sheahan, Richard A. Houghten, Jay P. McLaughlin, Jordan G. McCall, & Ream Al-Hasani Kappa opioid receptor potentiated cold hypersensitivity is transient receptor potential ankyrin 1-dependant in male mice but not in female mice Washington University School of Medicine, St. Louis, MO, USA
9:00 AM
Ellen S. Staedtler, Michael J. Iadarola, Matthew R. Sapio, Diana King, Dragan Maric, André
Ghetti, & Andrew J. Mannes Opioid receptor expression in human dorsal root ganglion: a perspective on peripheral opioid analgesia and alternative targets. National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA
9:20 AM
Mariana Spetea, Aina-Leonor Olivé-Marti, Alexandra Peer, Edin Muratspahić, Helmut Schmidhammer, & Christian W. Gruber Comparison of antinociceptive effects of structurally diverse, selective kappa-opioid receptor agonists in chronic inflammatory pain in mice University of Innsbruck, Innsbruck, Austria
9:40 AM
Paramita Basu & Bradley K. Taylor. Spinal kappa opioid receptor maintains latent postsurgical pain sensitization in a state of remission through tonic inhibition of a sex-specific, MEK→ ERK pronociceptive signaling pathway University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
10:00 AM Discussion
10:10 AM Coffee Break (with posters in room C1 / C2)
Oral Session 6: (Drug development, Ream Al-Hasani Chair)
10:30 AM
Jane V. Aldrich, Tanvir Khaliq, Shainnel Eans, Ariana C. Brice-Tutt, & Jay P. McLaughlin. Pharmacokinetics of macrocyclic tetrapeptide kappa opioid peptide antagonists and their potential for drug development. University of Florida, Gainesville, FL, USA
10:50 AM
Simone M. Creed, Anna M. Gutridge, Jillian L. Kyzer, J. Brent Friesen, Guido F. Pauli, Cody J. Wenthur, Richard M. van Rijn, Andrew P. Riley. Functionalization of Akuammicine – A Naturally Occurring Kappa Opioid Receptor Agonist University of Illinois, Chicago, IL, USA
11:10 AM
Christian W. Gruber. Design of nature-inspired macrocyclic peptide ligands for the κ-opioid receptor Medical University of Vienna, Vienna, Austria, USA
11:30 AM
Landsberg N, Guillot A, Riera-Calabuig, Cervera-Sospedra M, Polache A, Morón JA, Melero A, & Hipólito L. Development and characterization of a new nose-to-brain liposomal Pharmaceutical formulation targeting the kappa opioid receptor. University of Valencia, Spain
11:50 Discussion
12:00 PM -1:30 PM Buffet Lunch (rooms F1 / F2, G1 / G2)
Oral Session 7: (Dyn / KOR and reward function, Chair: Elyssa Margolis)
1:30 PM
Zahra Z. Farahbakhsh, Keaton Song, Hannah E. Branthwaite, Kirsty R. Erickson, Snigdha Mukerjee, Suzanne O. Nolan, & Cody A. Siciliano Systemic kappa opioid receptor antagonism accelerates reinforcement learning via augmentation of novelty processing in male mice Vanderbilt University, Nashville, TN, USA
1:50 PM
Wallace, C.W., Holleran, K.M., Centanni, S.W. & Jones, S.R. Kappa opioid receptors modulate real-time reward-related dopamine signaling in a sex-dependent manner Wake Forest University School of Medicine, Winston-Salem, NC, USA
2:10 PM
Valentina Martinez Damonte, & Julie A. Kauer.Kappa opioid control of a GABAergic stress-sensitive circuit involved in reinstatement. Stanford University, Palo Alto, CA, USA
2:30 PM
Aida Mohammadkhani, Min Qiao, & Stephanie L. Borgland. Effects of chronic morphine on LH orexin and dynorphin modulation of VTA dopamine neurons University of Calgary, Calgary, Canada
2:50 PM
Hector Bravo-Rivera, Aaron Limoges, Christina T LaGamma, & Hugo Tejeda. Dyn in vmPFC facilitates optimal approach/avoidance conflict resolution. National Institute of Mental Health, Bethesda, MD, USA
3:10 PM Discussion
Coffee Break 3:20 PM (with posters in room C1 / C2)
3:30 PM – 4:30 PM HOT Topics (Chair: Hugo Tejeda)
3:30 AM
Hector E. Yarur, Sanne M. Casello, Valerie Tsai, Juan Enriquez-Traba, Rufina Kore, Huikun Wang, Miguel Arenivar, & Hugo A. Tejeda. Dynorphin / kappa-opioid receptor regulation of excitation-inhibition balance toggles afferent control of prefrontal cortical circuits in a pathway-specific manner National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
3:40 PM
Bernard N. Johnson, Kiran Solingapuram Sai, Susan Nader, Songye Li, Yiyun Huang & Michael A. Nader. Kappa Opioid Receptor Availability, Social Rank, and Cocaine Self-Administration in Socially Housed Female and Male Monkeys Wake Forest University School of Medicine, Winston-Salem, NC, USA
3:50 PM
EJ Kuijer, SJ Bailey, DJ Heal, S Smith, S Wonnacott, & CP Bailey Electrophysiological analysis of kappa opioid receptor activation in mouse paraventricular thalamus University of Bath, Bath, UK
4:00 PM
Sofia Shirley, Hugo Tejeda, & Mario Penzo.Characterization of Endogenous Opioid Systems within the Paraventricular Nucleus of the Thalamus Johns Hopkins University and NIMH, Bethesda, MD, USA
4:10 – 5:00 PM Where do we go from here? A kappaphile community discourse on how to advance the field and increase translational impact (Moderator: Charles Chavkin)
5:00 – 6:00 PM Poster Session (room C1 / C2)
6:30-8:30 PM Closing Reception (wine & cheese)
(Bethesda Hyatt Rooftop Lounge)
7:30 PM Presentation of the 2023 Young Investigator Awards (Hugo Tejeda)
[mentors may nominate trainees by e-mail to H Tejeda; awards will be selected by vote of the Program Committee]
Dinner (no-host, maps to local restaurants provided)
Saturday, April 1st. Checkout & Departure
Poster Presentations
(Room C1 / C2; 4ft high x 6ft wide, pins provided)
1. Andrea Bedini, Elisabetta Cuna, Federica Santino, Luca Gentilucci, Santi Spampinato. RDM1127 is a novel, kappa opioid receptor (KOR)-selective ligand that displays a pharmacological profile suggestive of negative allosteric modulation. University of Bologna, Bologna, Italy
2. Dominika J. Burek, Mykel A. Robble, Andrew G. Hall, Elisa M. Taylor-Yeremeeva,
Eric J. Nestler, William A. Carlezon, Jr. Cell-type-specific regulation of Fosb gene expression in nucleus accumbens moderates effects of chronic stress on sleep and diurnal rhythms Harvard Medical School and McLean Hospital, Belmont, Massachusetts
3. Chongguang Chen, Kathryn Bland, Peng Huang, Conrad K. Ho, and Lee-Yuan Liu-Chen Kappa Opioid Receptor and Endogenous Ligand Visualized Together in a New Mouse Line: (Pdyn-iCre x Ai6) x KOR-tdTomato Temple University, Philadelphia, PA
4. Benjamin C. Coleman, Kevin M. Manz, Brad A. Grueter Kappa opioid receptor modulation of nucleus accumbens microcircuits Vanderbilt University, Nashville, TN
5. Monica Dawes, Katherine Holleran, Sara Jones Methamphetamine and fentanyl co- self-administration modifies fentanyl taking and exacerbates mesolimbic dopamine deficits Wake Forest School of Medicine, Winston-Salem, NC
6. Hastings, Lyndsay; Marchette, Renata; Frye, Emma; Carlson, Erika; Vendruscolo, Leandro; Koob, George F Extended kappa-opioid receptor antagonism reduces opioid self-administration in dependent mice National Institute on Drug Abuse and National Institute on Alcohol Abuse and Alcoholism Intramural Research Programs, Baltimore, MD
7. Peng Huang, Conrad K. Ho, Kathryn Bland, and Lee-Yuan Liu-Chen β-Arrestin 2 (arrb2) deletion has end-point dependent and sex-specific effects on the kappa opioid receptor (KOR)-mediated behaviors in mice Temple University, Philadelphia, PA
8. Michael J. Iadarola, Ellen S. Staedtler, Matthew R. Sapio, Diana M. King, Dragan Maric, André Ghetti, Kenneth A. Jacobson, Andrew J. Mannes Trans-species Transcriptomics of Receptors in Dorsal Root Ganglia to Identify Potential Analgesic Targets. NIHClinical Center, Bethesda, MD
9. Khairunisa Mohamad Ibrahim, Nicolas Massaly, Hye-Jean Yoon, Rossana Sandoval, Sidney Williams, Hannah Frye, William Post, Waylin Yu, Olayinka Idowu, Azra Zec, Sulan Pathiranage, Thomas L. Kash, Jose A. Morón Dorsal Hippocampus to Nucleus Accumbens Projections Drive Reinforcement Via Activation of Accumbal Dynorphin Neurons Washington University in St. Louis, St. Louis, MO
10. EJ Kuijer, SJ Bailey, DJ Heal, S Smith, S Wonnacott, CP Bailey Nalfurafine is aversive in doses that produce equi-effective antinociception to U50,488 University of Bath, Bath, UK
11. EJ Kuijer, SJ Bailey, DJ Heal, S Smith, S Wonnacott, CP Bailey A single injection of kappa opioid receptor agonist inhibits contextual heroin cues by counter-conditioning, not by enhancing extinction University of Bath, Bath, UK
12. C. Lebonville, H. Haun, W. Griffin, J. Rinker, P. Mulholland, and H. Becker Dissecting selective responses of dynorphin-expressing neurons during voluntary alcohol consumption in the central amygdala Medical University of South Carolina & VAMC, Charleston, SC
13. Aaron Limoges, Huikun Wang, Rodolfo Flores-Garcia, Hugo Tejeda Dynorphin signaling in the ventromedial prefrontal cortex regulates state transitions during acute threat exposure. National Institute of Mental Health, NIH, Bethesda MD
14. Jacob K. Meariman, Juan Gao Donald E. Mercante, Daniel R. Kapusta, Difelikefalin, a peripherally restricted KOR (kappa opioid receptor) agonist, produces diuresis through a central KOR pathway Louisiana State University, New Orleans, LA
15. Galen Missig, Sokhom Pin, Srinivas Chakilam, Sridhar Duvvuri, Philip Iredale Georgette L. Suidan CVL-354, a novel, brain penetrant and selective kappa opioid receptor antagonist Cerevel Therapeutics, Cambridge, MA
16. Siavash Shahbazi Nia, Guangchen Ji, Samuel Obeng, Ashrafur Rahman, Christopher R. McCurdy, Lance McMahon, Thomas J. Abbruscato, Paul C. Trippier, Volker Neugebauer,Nadezhda A. German Development of novel diketopiperazine and dipeptide analogs as selective KOR ligands as potential pain therapy Texas Tech University Health Sciences Center, Amarillo, Texas
17. Aaron J. Norris, Aaron L. Cone, Kenny K. Wu, and Alexxai V. Kravitz Kappa Opioid Receptor Activation Increases Energy Expenditure, Body Temperature, and Feeding Through Central Regulation of Brown Adipose Tissue Washington University in St. Louis, St. Louis, MO
18. Breanne E. Pirino, Brody A. Carpenter, Pelagia G. Candelas, Annie Hawks, Genevieve R. Curtis, Andrew T. Gargiulo, Anushree N. Karkhanis, & Jessica R. Barson Effects of the kappa-opioid receptor in the nucleus accumbens shell on ethanol drinking: Influence of sex, subregion targeted, and prior ethanol intake Drexel University College of Medicine, Philadelphia, PA
19. Sho Oasa, Erdinc Sezgin, Yuelong Ma, David A. Horne, Mihajlo Radmilović, Tijana Jovanović-Talisman, Rémi Martin-Fardon, Vladana Vukojević, Lars Terenius Live-cell time-resolved fluorescence microscopy/spectroscopy assess ethanol and kappa-opioid receptor (KOP) antagonists effect on KOP and lipid dynamics Karolinska Institutet, Stockholm, Sweden
20. Georgette L. Suidan, Megan Neal, Gillian Driscoll, Philip Iredale, Sridhar Duvvuri, Srinivas Chakilam, Giri Gokulrangan, Scott Carrier, Elena Chartoff A novel, short-acting kappa opioid receptor antagonist blocks the analgesic effects of U50,488 and attenuates symptoms of spontaneous oxycodone withdrawal in rats. Cerevel Therapeutics and McLean Hospital, Harvard Medical School, Boston, MA
21. Gavin J. Vaughan, Laura Navarro Gomez, Chelsea M. McNamara, Jessica R. Barson, Anushree N. Karkhanis Kappa opioid receptor control over monoaminergic transmission is differentially modulated by ethanol consumption along the NAc shell rostral-caudal axis Binghamton University, Binghamton, NY
22. Alyssa West, Katherine Holleran, Sara Jones Kappa opioid receptors reduce serotonin uptake and escitalopram efficiency in the substantia nigra pars reticulata Wake Forest University School of Medicine, Winston-Salem, USA